Usually means: Graphical interface for user interaction.
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We found 52 dictionaries that define the word GUI:

General (23 matching dictionaries)
  1. GUI, Gui: Merriam-Webster
  2. GUI: Oxford Learner's Dictionaries
  3. GUI: American Heritage Dictionary of the English Language
  4. GUI: Collins English Dictionary
  5. GUI: Vocabulary.com
  6. Gui, gui: Wordnik
  7. GUI, gui: Cambridge Advanced Learner's Dictionary
  8. GUI: Webster's New World College Dictionary, 4th Ed.
  9. Gui: Infoplease Dictionary
  10. GUI, gui: Dictionary.com
  11. GUI, Gui (ancestral surname), Gui (disambiguation), Gui (food), Gui (surname), Gui (vessel), Gui: Wikipedia, the Free Encyclopedia
  12. Gui: Rhymezone
  13. GUI: Stammtisch Beau Fleuve Acronyms
  14. Gui: Dictionary of Phrase and Fable (1898)
  15. gui: FreeDictionary.org
  16. gui: Mnemonic Dictionary
  17. GUI: TheFreeDictionary.com
  18. GUI: Wikimedia Commons US English Pronunciations
  19. gui: Longman Dictionary of Contemporary English
  20. GUI: Merriam-Webster
  21. GUI: Wiktionary

Art (1 matching dictionary)
  1. ODLIS: Online Dictionary of Library and Information Science (No longer online)

Computing (16 matching dictionaries)
  1. GUI: Free On-line Dictionary of Computing
  2. GUI: Netlingo
  3. GUI: CCI Computer
  4. GUI: Game Dictionary
  5. Technology Terms and Acronyms (No longer online)
  6. BABEL: Computer Oriented Abbreviations and Acronyms (No longer online)
  7. CNET Internet Glossary (No longer online)
  8. GUI: Computer Telephony & Electronics Dictionary and Glossary
  9. Glossary of Internet Terms (No longer online)
  10. GUI: Tech Terms Computer Dictionary
  11. Internet Terms (No longer online)
  12. Internet Terms (No longer online)
  13. Karbo's Dictionary (No longer online)
  14. Data Formats and Their Sugggested File Extensions (No longer online)
  15. I T Glossary (No longer online)
  16. GUI: Encyclopedia

Medicine (1 matching dictionary)
  1. online medical dictionary (No longer online)

Miscellaneous (3 matching dictionaries)
  1. Gui: Eastern Tea
  2. GUI: Acronym Finder
  3. AbbreviationZ (No longer online)

Science (2 matching dictionaries)
  1. Archaeology Wordsmith (No longer online)
  2. GUI: Extragalactic Astronomy

Slang (1 matching dictionary)
  1. G.U.I, GUI: Urban Dictionary

Tech (5 matching dictionaries)
  1. GUI: Webster's New World Telecom Dictionary
  2. GUI: GEOGRAPHIC INFORMATION SYSTEMS, CARTOGRAPHY, AND REMOTE SENSING
  3. Glossary of video terms (No longer online)
  4. Rane Professional Audio Reference (No longer online)
  5. Sweetwater Music (No longer online)

(Note: See guis as well.)

Definitions from Wiktionary (
)
American English Definition British English Definition
noun:  (computing) Initialism of graphical user interface. [(computing) A type of user interface which allows people to interact with a computer through a metaphor of direct manipulation of graphical images and widgets in addition to text.]

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