Usually means: Dominant, reproductive female in hive.
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We found 17 dictionaries that define the word queen bee:

General (16 matching dictionaries)
  1. queen bee: Longman Dictionary of Contemporary English
  2. queen bee: Merriam-Webster
  3. queen bee: Oxford Learner's Dictionaries
  4. queen bee: Collins English Dictionary
  5. queen bee: Vocabulary.com
  6. Queen-bee, queen-bee, queen bee: Wordnik
  7. queen bee: Wiktionary
  8. queen bee: Infoplease Dictionary
  9. Queen bee, queen bee: Dictionary.com
  10. Queen Bee (aircraft), Queen Bee (band), Queen Bee (comics), Queen Bee (film), Queen Bee (graphic novel), Queen Bee (ship), Queen Bee, Queen bee (aircraft), Queen bee (disambiguation), Queen bee (sociology), Queen bee (subculture), Queen bee, The Queen Bee (Garrett story), The Queen Bee: Wikipedia, the Free Encyclopedia
  11. queen bee: Rhymezone
  12. queen bee: FreeDictionary.org
  13. queen bee: Mnemonic Dictionary
  14. queen bee: TheFreeDictionary.com

Slang (1 matching dictionary)
  1. queen bee: Green’s Dictionary of Slang

(Note: See queen_bees as well.)

Definitions from Wiktionary (queen bee)

noun:  A reproductive female (especially the only one) in a colony of bees.
noun:  (idiomatic, colloquial, derogatory) The most important or dominant woman in an organization or situation.
noun:  (figurative) A stem cell.

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