Usually means: Determining DNA sequence using fragmentation.
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We found 7 dictionaries that define the word shotgun sequencing:

General (2 matching dictionaries)
  1. shotgun sequencing: Wiktionary
  2. Shotgun sequencing: Wikipedia, the Free Encyclopedia

Medicine (4 matching dictionaries)
  1. Shotgun sequencing: MedTerms.com Medical Dictionary
  2. Molecular Biology Glossary (No longer online)
  3. Shotgun sequencing: Medical dictionary
  4. Shotgun sequencing: Drug Medical Dictionary

Science (1 matching dictionary)
  1. Glossary of Genetic Terms (No longer online)

Definitions from Wiktionary (shotgun sequencing)

noun:  (genetics) A DNA sequencing technique in which a large number of small fragments of a long DNA strand are generated at random, sequenced, and reassembled to form a sequence of the original strand.

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