Usually means: Illumination technique, visible light microscopy.
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We found 3 dictionaries that define the word brightfield:

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  1. brightfield: Wordnik
  2. brightfield: Wiktionary
  3. brightfield: Dictionary.com

Definitions from Wiktionary (Brightfield)

noun:  Alternative spelling of bright field [Used to describe the most common form of optical microscopy in which light is transmitted through the sample which appears on a bright background]

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