Usually means: Sequential DNA sequencing using primers.
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We found 6 dictionaries that define the word primer walking:

General (2 matching dictionaries)
  1. Primer walking: Wikipedia, the Free Encyclopedia
  2. Primer walking: TheFreeDictionary.com

Business (2 matching dictionaries)
  1. Primer walking, Primer walking: Legal dictionary

Computing (1 matching dictionary)
  1. Primer walking: Encyclopedia

Medicine (1 matching dictionary)
  1. Primer walking: Medical dictionary

Definitions from Wikipedia (Primer walking)

noun:  a technique used to clone a gene (e.g., disease gene) from its known closest markers (e.g., known gene).

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