Sorry, no online dictionaries contain the exact phrase opposite of gladden.
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Reverse dictionary results:
1. elate
2. glad
3. joy
4. animate
5. buoy
6. cheer
7. cheering
8. cheers
9. lighten
10. please
11. aide
12. aides
13. allen
14. barker
15. charges
16. sad
17. enliven
18. gladdened
19. gladdening
20. blithe
21. cherish
22. cherishing
23. enlivened
24. enlivening
25. exhilarate
26. rejoice
27. anderson
28. appears
29. bartlett
30. bauer
31. beecher
32. bennett
33. blanco
34. breckinridge
35. brooke
36. campbell
37. clark
38. cole
39. coleman
40. cooper

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You can look up the words in the phrase individually using these links:   opposite ?   of ?   gladden ?
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